EphA2及EphrinA2在敖汉细毛羊皮肤中的表达及分布规律研究

doi:10.11843/j.issn.0366-6964.2016.03.012

Abstract

Abstract:

The objectives of the study were to identify the expression and distribution of EphA2 and EphrinA2 gene in hair follicles development and morphogenesis of Fine Wool sheep. The results might lay a theoretical basis for understanding molecular regulation mechanism of hair follicle development. Skins of Aohan Fine Wool sheep in the different period (prenatal stage, E90 d and E120 d; after birth, 1 d and 30 d) were selected. The qRT-PCR and immunohistochemical technologies were used to study their levels of mRNA and protein. The qRT-PCR results showed that mRNA expression level of EphA2 and EphrinA2 gene in E120 d were significantly higher than that of E90 d and 1 d (P＜0.01)，but 1 d and 30 d were not significantly difference (P＞0.05). Immunohistochemical detection results showed that, the level and localization of EphA2 and EphrinA2 protein were spatial and temporal specificity among E90 d, E120 d, 1 d and 30 d of sheep skin body side. And they mainly expressed in hair follicle, hair matrix outer root sheath and epidermis. In conclusion, EphA2 and EphrinA2 may play important roles in hair follicle development and morphogenesis. These results would provide fundamental information for the related research fields.

CLC Number:

S826.2

HE Jian-ning，WANG Chun-liang，ZHAO Jin-shan，CHENG Ming，LIU Kai-dong，LIU Ji-feng，LIU Nan. Expression and Distribution of EphA2 and EphrinA2 in Skin of Aohan Fine Wool Sheep[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(3): 509-514.

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Expression and Distribution of EphA2 and EphrinA2 in Skin of Aohan Fine Wool Sheep

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